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The effect of corticosteroids on lipolysis of rat adipocytes incubated in vitro was investigated with a closed system in which cell free medium was exchanged for fresh medium every 2 h during a 72 h incubation period. Dexamethasone (0.1, 1, 5.0 and 10.0 microM) increased glucose consumption in a dose dependent manner. The stimulatory action of dexamethasone was abolished by cycloheximide, indicating an involvement of protein synthesis in the effect. Basal and isoproterenol stimulated glycerol release were decreased by 1.0 and 5.0 microM dexamethasone and by 0.1 and 1.0 microM corticosterone. However, 10.0 microM dexamethasone, 10.0 microM corticosterone or 0.1 microM corticosterone significantly increased basal glycerol release. The stimulatory action of dexamethasone on glycerol release was abolished by 0.01 microM actinomycin D. The results show that the inhibitory action of corticosteroids on lipolysis is mediated by protein synthesis and may be interpreted as a regulatory mechanism to attenuate excessive lipolysis in the intact adipocyte.[Vasodilatory effect of a new antidepressant drug on the isolated femoral artery of the rat (author’s transl)].
The vasodilatory effect of a new antidepressant drug (Fluoxetine) on isolated femoral arteries from normotensive rats (Wistar) and spontaneously hypertensive rats (SHR) was studied. Fluoxetine induced a dose-dependent potent vasodilation in both groups of rats. The direct effect on the vascular smooth muscle was assessed. Its dose-response curve was similar in both groups of rats, with a
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